Journal: International Journal of Molecular Sciences

Article Title: Dual Oxidase 2 (DUOX2) as a Proteomic Biomarker for Predicting Treatment Response to Chemoradiation Therapy for Locally Advanced Rectal Cancer: Using High-Throughput Proteomic Analysis and Machine Learning Algorithm

doi: 10.3390/ijms232112923

Figure Lengend Snippet: ( A ) Machine learning approaches to measure the predictive power of proteins for CCRT response. ( B ) Immunohistochemical (IHC) validation of dual oxidase 2 (DUOX2) as a predictive biomarker for CCRT response (statistical significance, * p -value < 0.05).

Article Snippet: The monoclonal mouse anti-DUOX2 antibody (NB110-61576, Novus Biologicals, Centennial, CO, USA) was diluted to 1:100.

Techniques: Immunohistochemical staining, Biomarker Discovery

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: Pedigrees and variants of 3 unrelated families carrying DUOX2 variants. Pedigrees of family 1(A), 2(B), and 3(C). The genotype and phenotype information are displayed, +/− represents a heterozygous nonsense (c.1588A>T; p.K530X) variant in the DUOX2 gene, and −/− represents the wild type. (D) The four DUOX2 variants in these 3 families were validated by Sanger sequencing. Their locations are depicted in a corresponding lollipop plot of DUOX2 (created with IBS) and the conserved domains of the hDuox2 protein are also shown (E).

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Variant Assay, Sequencing

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: The truncated protein, hDuox2 K530, is overexpressed in adenomas. (A) Immunohistochemistry (IHC) staining of hDuox2 with an antibody targeting the N-terminal 1–100 amino acids of human Duox2 protein in the adenoma and normal tissues from a DUOX2 p.K530X carrier. The expression of non-phospho (active) β-Catenin was also detected in these 2 samples by IHC analysis. (B) The positions of the amino acid sequence recognized by 2 different anti-Duox2 antibodies are marked. (C) Immunofluorescence staining of wild-type and truncated proteins of hDuox2 in adenomas and normal tissues from a DUOX2 p.K530X carrier as well as the tumor from a DUOX2 wild-type patient. AA, amino acid.

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Immunohistochemistry, Expressing, Sequencing, Immunofluorescence, Staining

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: Overexpression of truncated protein, hDuox2 K530, promotes cell proliferation through the unfolded protein response. (A) Construction of the RKO cell line with stable expression of hDuox2 K530. (B) Growth curve of RKO-hDuox2 K530 and the control group. **** P < 0.0001 (C) Confocal microscopy with anti-6 × His-tag (red) and anti-calreticulin (green) in the RKO-hDuox2 K530 cell line. (D) Immunohistochemistry staining (100×) of GRP78 in the adenoma and normal tissues from a DUOX2 p.K530X carrier. EV, empty vector.

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Over Expression, Expressing, Confocal Microscopy, Immunohistochemistry, Staining, Plasmid Preparation

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: Schematic diagram of truncated protein, hDuox2 K530, causing adenomatous polyposis. The diagram shows that the truncated protein, hDuox2 K530, is translated due to a nonsense variant (c.1588A>T) occurring in the DUOX2 gene. Therefore, this misfolded protein is retained in the endoplasmic reticulum and activates an unfolded protein response, which could induce abnormal cell proliferation.

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Variant Assay